Human embryos cloned

[URL]http://www.environmentalgraffiti.com/sciencetech/human-embryos-cloned/[/URL]

at a loss for words

Re: Wow!

^^^ why is this sad? Being able to clone embryo's for someone so they can have healthy stem cells or to use for curing terminal diseases is a good thing no?

Re: Wow!

Read recently that they're managing to harvest stem cells without harming embryos anyway. If that is confirmed and becomes standard practice, a lot of the ethical issues will be sidestepped. But yeah, I'm all for it.

Re: Wow!

[quote=DancingQueen;561411]^^^ why is this sad? Being able to clone embryo's for someone so they can have healthy stem cells or to use for curing terminal diseases is a good thing no?[/quote]

not sad, (wasn't suppose to be sad, my bad) was amazed.

Re: Wow!

This is very exciting stuff. If this is validated, this is probably bigger than the discovery of Penicillin in terms of what it can do for medicine and treatment of disease. If anyone wants to read the actual article describing the research, the link is below. Its an open source article so you should be able to access it. If not, PM me for the PDF.

[URL]http://stemcells.alphamedpress.org.proxy1.lib.tju.edu:2048/cgi/content/full/26/1/119[/URL]

[B] Creation of Engineered Human Embryonic Stem Cell Lines Using phiC31 Integrase[/B]

ABSTRACT:

It has previously been shown that the phage-derived phiC31 integrase can efficiently target native pseudo-attachment sites in the genome of various species in cultured cells, as well as in vivo. To demonstrate its utility in human embryonic stem cells (hESC), we have created hESC-derived clones containing expression constructs. Variant human embryonic stem cell lines BG01v and SA002 were used to derive lines expressing a green fluorescent protein (GFP) marker under control of either the human Oct4 promoter or the EF1[IMG]http://stemcells.alphamedpress.org.proxy1.lib.tju.edu:2048/math/alpha.gif[/IMG] promoter. Stable clones were selected by antibiotic resistance and further characterized. The frequency of integration suggested candidate hot spots in the genome, which were mapped using a plasmid rescue strategy. The pseudo-[I]attP[/I] profile in hESC differed from those reported earlier in differentiated cells. [B]Clones derived using this method retained the ability to differentiate into all three germ layers,[/B] and fidelity of expression of GFP was verified in differentiation assays. GFP expression driven by the Oct4 promoter recapitulated endogenous Oct4 expression, whereas persistent stable expression of GFP expression driven by the EF1[IMG]http://stemcells.alphamedpress.org.proxy1.lib.tju.edu:2048/math/alpha.gif[/IMG] promoter was seen. Our results demonstrate the utility of phiC31 integrase to target pseudo-[I]attP[/I] sites in hESC and show that integrase-mediated site-specific integration can efficiently create stably expressing engineered human embryonic stem cell clones. Disclosure of potential conflicts of interest is found at the end of this article.

Re: Wow!

[quote=rainman;561620]not sad, (wasn't suppose to be sad, my bad) was amazed.[/quote]

the blue sad face you used on the initial post looks :( not :D

Re: Wow!

It's hard to define my feelings on this. This is definitely new terrortory for US humans. I behind it. I say go forth and Clone. It will ultimately benefit the human race, if in a trickle down kind of way. The thing about GEneticists is they have a hard time drawing the line in the moral sand. Nothing is sacred in the pursiut of knowledge.

Re: Wow!

didn't Dolly the sheep die of cancer tho at an early age?

long way off before this becomes usable? I would guess.

Re: Wow!

for US americans?

Re: Wow!

[quote=Steve Graham;562273]for US americans?[/quote]

+1?

otherwise i pretty much lead my life in hope of such progress :D

Re: Wow!

damn it, never mind

Re: Wow!

[quote=Steve Graham;561963]the blue sad face you used on the initial post looks :( not :D[/quote]

i know, i know, but could not edit that after i submitted post. :oops:

Re: Wow!

^^ Not your fault. ;) That's one of the x-files of the vbulletin software we are running for this forum..why you can't edit the icon you have chosen for the thread is something I never really understood.